serum protein free cell freezing medium  (MedChemExpress)

Journal: International Journal of Biological Sciences

Article Title: TCF7L2 promotes anoikis resistance and metastasis of gastric cancer by transcriptionally activating PLAUR

doi: 10.7150/ijbs.69933

Figure Lengend Snippet: TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and 293T cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.

Article Snippet: Lysis buffer was used to extract MKN45 and 293T cell total protein, and a BCA kit (Solarbio, Beijing, China) was used to measure the protein concentration.

Techniques: Confocal Microscopy, Expressing, Fluorescence, Quantitative RT-PCR, Western Blot, Binding Assay, ChIP-qPCR, Luciferase, Activity Assay, Mutagenesis, Control